| Western blot (WB): | 1:1000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |

Western blot analysis of anti-IDO2 antibody (M06002-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDO2 antigen affinity purified monoclonal antibody (M06002-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDO2 at approximately 47 kDa. The expected band size for IDO2 is at 45 kDa.

Immunofluorescent staining of HeLa cells using anti-IDO2 mouse monoclonal antibody.

Western blot analysis of anti-IDO2 antibody (M06002-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IDO2 antigen affinity purified monoclonal antibody (M06002-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IDO2 at approximately 47 kDa. The expected band size for IDO2 is at 45 kDa.

Immunofluorescent staining of HeLa cells using anti-IDO2 mouse monoclonal antibody.

