Anti-UBC12/UBE2M Antibody (Clone#OTI2D9)

Mouse monoclonal antibody

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M07478

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2M monoclonal antibody.
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Anti-UBE2M mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2M(Cat# M07478).
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产品名称

Anti-UBC12/UBE2M Antibody (Clone#OTI2D9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hUbc12; NEDD8 carrier protein; NEDD8 conjugating enzyme Ubc12; NEDD8 protein ligase; UBC RS2; UBC12; UBE2M

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, ICC/IF

基因名称

UBE2M

克隆号

OTI2D9

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human UBE2M(NP_003960) produced in HEK293T cell.

计算分子量

21 kDa

实际分子量

21 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq]

Uniprot ID

P61081

文献引用格式

UBC12/UBE2M Antibody (Clone#OTI2D9) (Boster Biological Technology, Wuhan, China. Catalog#M07478)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ubiquitin conjugating enzyme E2 M

蛋白名全称

NEDD8-conjugating enzyme Ubc12

实验方案>

实验步骤

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