Anti-HGF Antibody (Clone#OTI1D2)

Mouse monoclonal antibody

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筛选器： All IHC WB

MA00089

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00089) (1:150)
all(7)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGF (1:2000).
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HGF Antibody (Clone#OTI1D2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

F TCF; Hepatocyte growth factor; Hepatopoeitin A; HGF; HGFB; HPTA; Scatter factor; SF

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

HGF

克隆号

OTI1D2

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 200-494 of human HGF(NP_000592) produced in E.coli.

计算分子量

83 kDa

实际分子量

79.6 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. This gene is mapped to 7q21.11. It is secreted by mesenchymal cells and targets and acts primarily uponepithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration and in wound healing. HGF can regulate cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF also serves as a paracrine mediator to control placental development and growth. HGF was identified as one of the liver sinusoidal endothelial cell-derived paracrine mediators promoting hepatocyte growth.

Uniprot ID

Q08048

文献引用格式

HGF Antibody (Clone#OTI1D2) (Boster Biological Technology, Wuhan, China. Catalog#MA00089)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

hepatocyte growth factor (hepapoietin A; scatter factor)

蛋白名全称

Hepatocyte growth factor

实验方案>

实验步骤

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