Anti-ALOX12 Antibody (Clone#OTI1C3)

Mouse monoclonal antibody

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筛选器： All WB IHC

MA02275

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX12 (Cat# M02275)(1:2000).
all(3)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M02275) (1:150)
all(3)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ALOX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M02275) (1:150)
all(3)

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产品简介>

产品名称

Anti-ALOX12 Antibody (Clone#OTI1C3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Arachidonate (12S)-lipoxygenase; 12-LOX; 12S-LOX; 12S-lipoxygenase; Arachidonate (15S)-lipoxygenase; Linoleate (13S)-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12; ALOX12; 12LO; LOG12

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

ALOX12

克隆号

OTI1C3

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human ALOX12 (NP_000688) produced in HEK293T cell.

计算分子量

76 kDa

实际分子量

75.5 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

Uniprot ID

P18054

文献引用格式

ALOX12 Antibody (Clone#OTI1C3) (Boster Biological Technology, Wuhan, China. Catalog#MA02275)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 12-lipoxygenase

蛋白名全称

Arachidonate 12-lipoxygenase, 12S-type(12S-LOX/12S-lipoxygenase)

实验方案>

实验步骤

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