Anti-SMAD4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0446

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SMAD4 using anti-SMAD4 antibody (PB0446). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Mouse Brain tissue lysates,
Lane 3: Rat Skeletal Muscle tissue lysates,
Lane 4: Mouse Skeletal Muscle tissue lysates,
Lane 5: U87 whole cell lysates,
Lane 6: Human Placenta tissue lysates,
Lane 7: HT1080 whole cell lysates,
Lane 8: HELA whole cell lysates,
Lane 9: NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD4 antigen affinity purified polyclonal antibody (PB0446) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD4 at approximately 60 kDa. The expected band size for SMAD4 is at 60 kDa.
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产品简介>

产品名称

Anti-SMAD4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DPC4; hSMAD4; JIP; MAD homolog 4; MADH4; Mothers against DPP homolog 4; SMAD 4; SMAD family member 4; SMAD4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

SMAD4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4 identical to the related mouse and rat sequences.

计算分子量

60 kDa

实际分子量

60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFβ protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

Uniprot ID

Q13485

文献引用格式

SMAD4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0446)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 4

蛋白名全称

Mothers against decapentaplegic homolog 4

实验方案>

实验步骤

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