Anti-ATP1A1 Antibody

Rabbit Polyclonal Antibody

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筛选器： All WB IHC

PB0504

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue (37℃) lysates,
Lane 2: rat brain tissue (100℃) lysates,
Lane 3: mouse brain tissue (37℃) lysates,
Lane 4: mouse brain tissue (100℃) lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified polyclonal antibody (PB0504) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATP1A1 at approximately 100 kDa. The expected band size for ATP1A1 is at 113 kDa.
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IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB0504) .
ATP1A1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-ATP1A1 Antibody (PB0504) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATP1A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ATP1A1; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha 1 subunit; Na+/K+ ATPase 1; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump subunit alpha 1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ATP1A1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1 different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.

计算分子量

113 kDa

实际分子量

100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P05023

文献引用格式

ATP1A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0504)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATPase, Na+/K+ transporting, alpha 1 polypeptide

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