Anti-Gremlin/GREM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0667

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Gremlin/GREM1 using anti-Gremlin/GREM1 antibody (PB0667). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gremlin/GREM1 antigen affinity purified polyclonal antibody (PB0667) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Gremlin/GREM1 at approximately 21 kDa. The expected band size for Gremlin/GREM1 is at 21 kDa.
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产品简介>

产品名称

Anti-Gremlin/GREM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

gremlin 1; DAN family BMP antagonist; C15DUPq; CKTSF1B1; CRAC1; CRCS4; DAND2; DRM; DUP15q; GREMLIN; HMPS; HMPS1; IHG-2; MPSH; PIG2; GREM1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

GREM1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Gremlin 1 identical to the related mouse and rat sequences.

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Gremlin, also known as Drm, is a highly conserved 20.7-kDa, 184 amino acid glycoprotein part of the DAN family and is a cysteine knot-secreted protein. Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. And Gremlin is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. Gremlin 1 (GREM 1) is known for its antagonistic reaction with BMPs in the TGF beta signaling pathway. This gene inhibits BMP-2, BMP-4, and BMP-7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. Gremlin 1 may play an oncogenic role especially in carcinomas of the uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma. Over-expressed gremlin 1 functions by interaction with YWHAH (Its binding site for gremlin 1 was located between residues 61-80 and gremlin 1 binding site for YWHAH was found to be located between residues 1-67). Therefore, Gremlin 1 and its binding protein YWHAH could be good targets for developing diagnostic and therapeutic strategies against human cancers.

Uniprot ID

O60565

文献引用格式

Gremlin/GREM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0667)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

gremlin 1

蛋白名全称

Gremlin-1

实验方案>

实验步骤

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