Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of FGF19 using anti-FGF19 antibody (PB1116). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (PB1116) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
Western blot analysis of FGF19 using anti-FGF19 antibody (PB1116). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (PB1116) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.