Anti-HLTF Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of HLTF using anti-HLTF antibody (PB1124). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human PANC-1 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: rat heart tissue lysates,
  Lane 5: rat kidney tissue lysates,
  Lane 6: mouse heart tissue lysates,
  Lane 7: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (PB1124) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.

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  IF analysis of HLTF using anti-HLTF antibody (PB1124) and anti-Alpha Tubulin antibody (M03989-3).
  HLTF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HLTF Antibody (PB1124) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.

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  Flow Cytometry analysis of Caco-2 cells using anti-HLTF antibody (PB1124).
  Overlay histogram showing Caco-2 cells stained with PB1124 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLTF Antibody (PB1124) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of HLTF using anti-HLTF antibody (PB1124). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human PANC-1 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: rat heart tissue lysates,
  Lane 5: rat kidney tissue lysates,
  Lane 6: mouse heart tissue lysates,
  Lane 7: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (PB1124) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.

• 

  IF analysis of HLTF using anti-HLTF antibody (PB1124) and anti-Alpha Tubulin antibody (M03989-3).
  HLTF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-HLTF Antibody (PB1124) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.

• 

  Flow Cytometry analysis of Caco-2 cells using anti-HLTF antibody (PB1124).
  Overlay histogram showing Caco-2 cells stained with PB1124 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLTF Antibody (PB1124) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.