Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of Mast Cell Chymase/CMA1 using anti-Mast Cell Chymase/CMA1 antibody (PB9055).
Mast Cell Chymase/CMA1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Mast Cell Chymase/CMA1 Antibody (PB9055) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of Mast Cell Chymase/CMA1 using anti-Mast Cell Chymase/CMA1 antibody (PB9055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Mast Cell Chymase/CMA1 antigen affinity purified polyclonal antibody (PB9055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Mast Cell Chymase/CMA1 at approximately 27-35 kDa. The expected band size for Mast Cell Chymase/CMA1 is at 27 kDa.
IHC analysis of Mast Cell Chymase/CMA1 using anti-Mast Cell Chymase/CMA1 antibody (PB9055).
Mast Cell Chymase/CMA1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Mast Cell Chymase/CMA1 Antibody (PB9055) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of Mast Cell Chymase/CMA1 using anti-Mast Cell Chymase/CMA1 antibody (PB9055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Mast Cell Chymase/CMA1 antigen affinity purified polyclonal antibody (PB9055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Mast Cell Chymase/CMA1 at approximately 27-35 kDa. The expected band size for Mast Cell Chymase/CMA1 is at 27 kDa.