Anti-RUNX1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9157

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RUNX1 using anti-RUNX1 antibody (PB9157). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX1 antigen affinity purified polyclonal antibody (PB9157) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RUNX1 at approximately 49 kDa. The expected band size for RUNX1 is at 49 kDa.
all(6)
IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in a paraffin-embedded section of rat thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RUNX1 Antibody (PB9157) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RUNX1 Antibody (PB9157) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RUNX1 Antibody (PB9157) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RUNX1 Antibody (PB9157) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in frozen section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RUNX1 Antibody (PB9157) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)

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产品简介>

产品名称

Anti-RUNX1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AML1; AML1 EVI 1; AMLCR1; CBF alpha 2; CBFA2; EVI 1; Oncogene AML 1; PEA2 alpha B; PEBP2 alpha B; PEBP2aB; RUNX1; RUNX1 (middle)

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

RUNX1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human RUNX1, identical to the related mouse and rat sequences.

计算分子量

49 kDa

实际分子量

49 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Runt-related transcription factor 1 (RUNX1), also known as AML1 or CBFA2, is a protein that in humans is encoded by the RUNX1 gene. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). RUNX1 is mapped to 21q22.12. RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. Mutations in RUNX1 are implicated in cases of breast cancer.

Uniprot ID

Q01196

文献引用格式

RUNX1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9157)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

runt-related transcription factor 1

蛋白名全称

Runt-related transcription factor 1

实验方案>

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