Anti-Decorin/DCN Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

PB9174

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174).
Lane 1: recombinant human Decorin protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (PB9174) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 38 kDa.
all(3)
Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SW620 whole cell lysates,
Lane 7: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (PB9174) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 40 kDa. The expected band size for Decorin/DCN is at 40 kDa.
all(3)
IHC analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174).
Decorin/DCN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Decorin/DCN Antibody (PB9174) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Decorin/DCN Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Bone proteoglycan II; CSCD; DCN; Decorin; DSPG2; PG S2; PG40; PGII; PGS2; SLRR1B

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

DCN

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Decorin recombinant protein (Position: D31-K359). Human Decorin shares 80% and 77% amino acid (aa) sequences identity with mouse and rat Decorin, respectively.

计算分子量

40 kDa

实际分子量

40 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Decorin is a protein that in humans is encoded by the DCN gene. This gene is mapped to 12q21.3. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping. Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines.

Uniprot ID

P07585

RRID

AB_3082002

文献引用格式

Decorin/DCN Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9174)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

decorin

蛋白名全称

Decorin

实验方案>

实验步骤

引用文献(0)>

清除