Anti-YY1 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of YY1 using anti-YY1 antibody (PB9909). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse thymus tissue lysates,
  Lane 2: mouse spleen tissue lysates,
  Lane 3: rat thymus tissue lysates,
  Lane 4: pc-12 whole cell lysates,
  Lane 5: RAW264.7 whole cell lysates,
  Lane 6: PC-3 whole cell lysates,
  Lane 7: Sw620 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YY1 antigen affinity purified polyclonal antibody (PB9909) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 45 kDa.

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  IF analysis of YY1 using anti-YY1 antibody (PB9909).
  YY1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-YY1 Antibody (PB9909) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Flow Cytometry analysis of THP-1 cells using anti-YY1 antibody (PB9909).
  Overlay histogram showing THP-1 cells stained with PB9909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YY1 Antibody (PB9909) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of YY1 using anti-YY1 antibody (PB9909). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse thymus tissue lysates,
  Lane 2: mouse spleen tissue lysates,
  Lane 3: rat thymus tissue lysates,
  Lane 4: pc-12 whole cell lysates,
  Lane 5: RAW264.7 whole cell lysates,
  Lane 6: PC-3 whole cell lysates,
  Lane 7: Sw620 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YY1 antigen affinity purified polyclonal antibody (PB9909) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 45 kDa.

• 

  IF analysis of YY1 using anti-YY1 antibody (PB9909).
  YY1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-YY1 Antibody (PB9909) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Flow Cytometry analysis of THP-1 cells using anti-YY1 antibody (PB9909).
  Overlay histogram showing THP-1 cells stained with PB9909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YY1 Antibody (PB9909) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.