Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB IHC FCM ELISA

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  Figure 1. Western blot analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat skeletal muscle tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse skeletal muscle tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific antigen affinity purified polyclonal antibody (A04148-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 GAMMA/YWHAG-Specific at approximately 28 kDa. The expected band size for 14-3-3 GAMMA/YWHAG-Specific is at 28 kDa.

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  Figure 2. IHC analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2).14-3-3 GAMMA/YWHAG-Specific was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of U87 cells using anti-14-3-3GAMMA/YWHAG-Specific antibody (A04148-2).
  Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Human ARMETL1/CDNF ELISA Kit

Anti-Beta Actin/ACTB Antibody

筛选器： All WB

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  Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human Jurkat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat kidney tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

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  Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: zebra fish tissue lysates,
  Lane 2: chicken heart tissue lysates,
  Lane 3: chicken liver tissue lysates,
  Lane 4: chicken brain tissue lysates,
  Lane 5: monkey heart tissue lysates,
  Lane 6: monkey lung tissue lysates,
  Lane 7: monkey kidney tissue lysates,
  Lane 8: monkey liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

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  Figure 3. Western blot analysis of Beta Actin/ACTB using anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human THP-1 whole cell lysates,
  Lane 3: rat liver tissue lysates,
  Lane 4: mouse liver tissue lysates,
  Lane 5: human Hela whole cell lysates,
  Lane 6: human THP-1 whole cell lysates,
  Lane 7: rat liver tissue lysates,
  Lane 8: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) at a dilution of 1:50000 (Lane1-4) and 1:100000 (Lane5-8) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Anti-Beta Actin/ACTB Antibody

筛选器： All WB IHC

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  Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

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  Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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  Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Anti-Beta Actin/ACTB Antibody (Clone#BF-1)

筛选器： All WB IHC ICC/IF

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  Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BM3873). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human GES-1 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: mouse PC-12 whole cell lysates,
  Lane 7: mouse Neuro-2a whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (BM3873) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Anti-GAPDH Antibody

筛选器： All WB IHC ICC/IF FCM

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  Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Caco-2 whole cell lysates,
  Lane 3: CCRF-CEM whole cell lysates,
  Lane 4: rat brain tissue lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (A00227-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Figure 2. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human THP-1 whole cell lysates,
  Lane 3: rat liver tissue lysates,
  Lane 4: mouse liver tissue lysates,
  Lane 5: human Hela whole cell lysates,
  Lane 6: human THP-1 whole cell lysates,
  Lane 7: rat liver tissue lysates,
  Lane 8: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (A00227-1) at a dilution of 1:50000 (Lane1-4) and 1:100000 (Lane5-8) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Figure 3. IHC analysis of GAPDH using anti-GAPDH antibody (A00227-1).
  GAPDH was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GAPDH Antibody (A00227-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of GAPDH using anti-GAPDH antibody (A00227-1).
  GAPDH was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GAPDH Antibody (A00227-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IF analysis of GAPDH using anti-GAPDH antibody (A00227-1).
  GAPDH was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-GAPDH Antibody (A00227-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 6. Flow Cytometry analysis of Hela cells using anti-GAPDH antibody (A00227-1).
  Overlay histogram showing Hela cells stained with A00227-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAPDH Antibody (A00227-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-GAPDH Antibody (Clone#5A12)

筛选器： All WB ICC/IF IP

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  Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human THP-1 whole cell lysates,
  Lane 5: human Hacat whole cell lysates,
  Lane 6: human HUVEC whole cell lysates,
  Lane 7: human SK-OV-3 whole cell lysates,
  Lane 8: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:10000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Figure 2. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat NRK whole cell lysates,
  Lane 3: mouse liver tissue lysates,
  Lane 4: mouse SP2/0 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:10000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Anti-GAPDH Antibody (Clone#BG-7)

筛选器： All WB IHC ICC/IF

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  Figure 1. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human PC-3 whole cell lysates,
  Lane 6: monkey COS-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Figure 2. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat RH-35 whole cell lysates,
  Lane 4: mouse liver tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

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  Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.

七色多重荧光染色试剂盒Plus(六标七色)

三色多重荧光染色试剂盒Plus(双标三色)

五色多重荧光染色试剂盒Plus(四标五色)

六色多重荧光染色试剂盒Plus(五标六色)

四色多重荧光染色试剂盒Plus(三标四色)

荧光单标染色信号放大试剂盒（红光）

荧光单标染色信号放大试剂盒（绿光）

0.02%EDTA溶液

0.05%胰酶溶液(不含EDTA)

0.05%胰酶溶液(含EDTA)

0.25%胰酶溶液(不含EDTA,含酚红)

0.25%胰酶溶液(不含EDTA)