Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.

Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate.

Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.

Western blot analysis of 14-3-3 sigma expression in A431 cell lysate.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

Western blot analysis of anti-YWHAQ antibody (BM4757). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YWHAQ antigen affinity purified monoclonal antibody (BM4757) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YWHAQ at approximately 28 kDa. The expected band size for YWHAQ is at 28 kDa.

IHC analysis of YWHAQ using anti-YWHAQ antibody (BM4757).
YWHAQ was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-YWHAQ Antibody (BM4757) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of anti-P-EIF4EBP1 antibody (M00968T70). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-EIF4EBP1 antigen affinity purified monoclonal antibody (M00968T70) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-EIF4EBP1 at approximately 17 kDa. The expected band size for P-EIF4EBP1 is at 17 kDa.

Western blot analysis of anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antibody (M00968T46T32). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) antigen affinity purified monoclonal antibody (M00968T46T32) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) at approximately 18 kDa. The expected band size for 4EBP1/EIF4EBP1/2/3(Phospho-T46/T46/T32) is at 18 kDa.

Western blot analysis of anti-5 Lipoxygenase/ALOX5 antibody (BM5522). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-5 Lipoxygenase/ALOX5 antigen affinity purified monoclonal antibody (BM5522) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 5 Lipoxygenase/ALOX5 at approximately 78 kDa. The expected band size for 5 Lipoxygenase/ALOX5 is at 78 kDa.

Western blot analysis of ASPP2 expression in MCF7 cell lysate.

Western blot analysis of anti-a-SMA/ACTA2 antibody (BM4172). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM4172) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U251 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Western blot analysis of anti-a-SMA/ACTA2 antibody (BM3902). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM3902) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

Western blot analysis of AAMP expression in (1) A375 lysate; (2) MCF7 cell lysate.

Western blot analysis of ABAT expression in HepG2 cell lysate.

Western blot analysis of MRP1 expression in A431 cell lysate.

Western blot analysis of ABCC2 using anti-ABCC2 antibody (BM5126). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HUH-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCC2 antigen affinity purified polyclonal antibody (BM5126) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCC2 at approximately 170-250 kDa. The expected band size for ABCC2 is at 174 kDa.

Western blot analysis of ABCD1 / ALD in HepG2 cell lysate.

Western blot analysis of PMP70 expression in HepG2 cell lysate.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

Western blot analysis of ABCE1 expression in HeLa cell lysate.

Western blot analysis of anti-ABCF1 antibody (BM4542). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCF1 antigen affinity purified monoclonal antibody (BM4542) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCF1 at approximately 110 kDa. The expected band size for ABCF1 is at 96 kDa.

Immunohistochemical analysis of paraffin-embedded human colon, using ABCF1 Antibody.

Western blot analysis of ABHD5 expression in HepG2 cell lysate.