Anti-STAT1 Antibody

筛选器： All WB FCM ELISA

• 

  Western blot analysis of anti- STAT1 antibody (A00036-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human PC-3 whole cell lysates.
  Use rabbit anti- STAT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.

• 

  Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T- WT whole cell lysates,
  Lane 2: human 293T-STAT1 KO whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (A00036-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.

• 

  Flow Cytometry analysis of HCT116 cells using anti-STAT1 antibody (A00036-3).
  Overlay histogram showing HCT116 cells stained with A00036-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (A00036-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 2°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- STAT1 antibody (A00036-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human PC-3 whole cell lysates.
  Use rabbit anti- STAT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.

• 

  Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T- WT whole cell lysates,
  Lane 2: human 293T-STAT1 KO whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (A00036-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.

• 

  Flow Cytometry analysis of HCT116 cells using anti-STAT1 antibody (A00036-3).
  Overlay histogram showing HCT116 cells stained with A00036-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (A00036-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 2°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•