Anti-CYP19A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00071-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CYP19A1 using anti-CYP19A1 antibody (A00071-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: HCCT tissue lysates,
Lane 4: HCCP tissue lysates,
Lane 5: U-87MG whole cell lysates,
Lane 6: MCF-7 whole cell lysates,
Lane 7: SK-OV-3 whole cell lysates,
Lane 8: SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP19A1 antigen affinity purified polyclonal antibody (A00071-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP19A1 at approximately 58 kDa. The expected band size for CYP19A1 is at 58 kDa.
all(3)
Western blot analysis of CYP19A1 using anti-CYP19A1 antibody (A00071-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse ovary tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP19A1 antigen affinity purified polyclonal antibody (A00071-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP19A1 at approximately 58 kDa. The expected band size for CYP19A1 is at 58 kDa.
all(3)
all(3)

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产品简介>

产品名称

Anti-CYP19A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase; CYP19A1; ARO1; CYAR; CYP19

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

CYP19A1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Aromatase/Cyp19a1 recombinant protein (Position: N75-Q428).

计算分子量

58 kDa

实际分子量

58 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

Uniprot ID

P28649

RRID

AB_3080928

文献引用格式

CYP19A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00071-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cytochrome P450, family 19, subfamily a, polypeptide 1

蛋白名全称

Aromatase

实验方案>

实验步骤

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