Anti-Hemoglobin/HBA1/HBA2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC FCM

A00233-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-Hemoglobin/HBA1/HBA2 antibody (A00233-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 antigen affinity purified polyclonal antibody (A00233-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Hemoglobin/HBA1/HBA2 at approximately 15 kDa. The expected band size for Hemoglobin/HBA1/HBA2 is at 15 kDa.
all(4)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-1).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-1).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Flow Cytometry analysis of K562 cells using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-1).
Overlay histogram showing K562 cells stained with A00233-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Hemoglobin/HBA1/HBA2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha globin; HBA1; HBA2; Hemoglobin a; Hemoglobin Alpha; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin; alpha 1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

HBA1/HBA2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Hemoglobin recombinant protein (Position: V2-R142). Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.

计算分子量

15 kDa

实际分子量

15 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Uniprot ID

P69905

文献引用格式

Hemoglobin/HBA1/HBA2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00233-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除