Anti-Hemoglobin/HBA1/HBA2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A00233-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 antigen affinity purified polyclonal antibody (A00233-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Hemoglobin/HBA1/HBA2 at approximately 15 kDa. The expected band size for Hemoglobin/HBA1/HBA2 is at 15 kDa.
all(5)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human speen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2).
Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Hemoglobin/HBA1/HBA2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha globin; HBA1; HBA2; Hemoglobin a; Hemoglobin Alpha; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin; alpha 1

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

HBA1/HBA2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Hemoglobin/HBA1/HBA2, identical to the related mouse and rat sequences.

计算分子量

15 kDa

实际分子量

15 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Uniprot ID

P69905

文献引用格式

Hemoglobin/HBA1/HBA2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00233-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

hemoglobin subunit alpha 1/2

蛋白名全称

Hemoglobin subunit alpha

实验方案>

实验步骤

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