Anti-CEBPA Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Western blot analysis of anti- CEBPA antibody (A00386-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates,
  Lane 2: human U-937 whole cell lysates,
  Lane 3: human HepG2 whole cell lysates.
  Use rabbit anti- CEBPA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CEBPA at approximately 42KD. The expected band size for CEBPA is at 37KD.

• 

  ICC/IF analysis of CEBPA using anti-CEBPA antibody (A00386-1).
  CEBPA was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-CEBPA Antibody (A00386-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Caco-2 cells using anti-CEBPA antibody (A00386-1).
  Overlay histogram showing Caco-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBPA Antibody (A00386-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- CEBPA antibody (A00386-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates,
  Lane 2: human U-937 whole cell lysates,
  Lane 3: human HepG2 whole cell lysates.
  Use rabbit anti- CEBPA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CEBPA at approximately 42KD. The expected band size for CEBPA is at 37KD.

• 

  ICC/IF analysis of CEBPA using anti-CEBPA antibody (A00386-1).
  CEBPA was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-CEBPA Antibody (A00386-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Caco-2 cells using anti-CEBPA antibody (A00386-1).
  Overlay histogram showing Caco-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBPA Antibody (A00386-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•