Anti-Cytokeratin 5/KRT5 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A00398

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- KRT5 antibody (A00398). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
Use rabbit anti- KRT5 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for KRT5 at approximately 62KD. The expected band size for KRT5 is at 62KD.
all(3)
IHC analysis of Cytokeratin 5/KRT5 using anti-Cytokeratin 5/KRT5 antibody (A00398).
Cytokeratin 5/KRT5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytokeratin 5/KRT5 Antibody (A00398) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of Cytokeratin 5/KRT5 using anti-Cytokeratin 5/KRT5 antibody (A00398).
Cytokeratin 5/KRT5 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytokeratin 5/KRT5 Antibody (A00398) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-Cytokeratin 5/KRT5 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

58 kDa cytokeratin; Cell and organelle markers; CK 5; CK5; Cytokeratin 5; Cytoskeleton Marker; K5; keratin 5; KRT5; KRT5A; Type II keratin Kb5

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

KRT5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

计算分子量

62 kDa

实际分子量

62 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Uniprot ID

P13647

RRID

AB_3081100

文献引用格式

Cytokeratin 5/KRT5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00398)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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