Western blot analysis of anti- NQO1 antibody (A00494-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
Use rabbit anti- NQO1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NQO1 at approximately 31KD. The expected band size for NQO1 is at 31KD.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of MCF-7 cells using anti-NQO1 antibody (A00494-2).
Overlay histogram showing MCF-7 cells stained with A00494-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NQO1 Antibody (A00494-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti- NQO1 antibody (A00494-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
Use rabbit anti- NQO1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NQO1 at approximately 31KD. The expected band size for NQO1 is at 31KD.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of MCF-7 cells using anti-NQO1 antibody (A00494-2).
Overlay histogram showing MCF-7 cells stained with A00494-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NQO1 Antibody (A00494-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of anti- NQO1 antibody (A00494-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates.
Use rabbit anti- NQO1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NQO1 at approximately 31KD. The expected band size for NQO1 is at 31KD.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of NQO1 using anti-NQO1 antibody (A00494-2).
NQO1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-NQO1 Antibody (A00494-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow Cytometry analysis of MCF-7 cells using anti-NQO1 antibody (A00494-2).
Overlay histogram showing MCF-7 cells stained with A00494-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NQO1 Antibody (A00494-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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