Anti-TRPV4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00565-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TRPV4 using anti-TRPV4 antibody (A00565-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (A00565-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRPV4 at approximately 98 kDa. The expected band size for TRPV4 is at 98 kDa.
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产品简介>

产品名称

Anti-TRPV4 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Osm 9 like TRP channel 4; OTRPC4; TRP12; TRPV4; VR OAC; VRL 2; VRL2; VROAC

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

TRPV4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human TRPV4 recombinant protein (Position: N66-L871).

计算分子量

98 kDa

实际分子量

98 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TRPV4(Transient Receptor Potential Cation Channel Subfamily V Member 4), also known as TRP12, OTRPC4 or VROAC, is a protein that in humans is encoded by the TRPV4 gene. This gene encodes TRPV4, a member of the OSM9-like transient receptor potential channel(OTRPC) subfamily in the transient receptor potential(TRP) superfamily of ion channels. By genomic sequence analysis, Liedtke et al.(2000) mapped the TRPV4 gene to chromosome 12q24.1. They mapped the mouse Trpv4 gene to distal chromosome 5 by radiation hybrid analysis. Wissenbach et al.(2000) found that hypoosmotic conditions rapidly activated TRP12, while hyperosmotic conditions inhibited the activity.Strotmann et al.(2000) showed that OTRPC4 was responsive to changes in extracellular osmolarity in the physiologically relevant range and was expressed in tissues exposed to changing osmotic environments.

Uniprot ID

Q9HBA0

文献引用格式

TRPV4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00565-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transient receptor potential cation channel subfamily V member 4

蛋白名全称

Transient receptor potential cation channel subfamily V member 4

实验方案>

实验步骤

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