Anti-CYP17A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ELISA

A00615-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CYP17A1 antigen affinity purified polyclonal antibody (A00615-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CYP17A1 at approximately 57 kDa. The expected band size for CYP17A1 is at 57 kDa.
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IHC analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-3).
CYP17A1 was detected in a paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP17A1 Antibody (A00615-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-3).
CYP17A1 was detected in a paraffin-embedded section of rat testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CYP17A1 Antibody (A00615-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- Cyp17a1 antibody (A00615-3). detected in paraffin-embedded section of mouse testis tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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产品简介>

产品名称

Anti-CYP17A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 C17; Cytochrome P450c17; P450C17; S17AH; Steroid 17 alpha monooxygenase

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, IF, ELISA

基因名称

CYP17A1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363). Mouse Cyp17a1 shares 65.6% and 78.2% amino acid (aa) sequence identity with human and rat Cyp17a1 respectively.

计算分子量

57 kDa

实际分子量

57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cytochrome P450 17A1, also called steroid 17α-monooxygenase, is an enzyme of the hydroxylase type that in humans is encoded by the CYP17A1 gene on chromosome 10. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Uniprot ID

P27786

文献引用格式

CYP17A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00615-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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