Western blot analysis of anti-GLI2 antibody (A00701-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (A00701-6) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 168 kDa.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GLI2 using anti-GLI2 antibody (A00701-6) and anti-Beta Tubulin antibody (M01857-3).
GLI2 was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of U2OS cells using anti-GLI2 antibody (A00701-6).
Overlay histogram showing U2OS cells stained with A00701-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (A00701-6, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-GLI2 antibody (A00701-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (A00701-6) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 168 kDa.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GLI2 using anti-GLI2 antibody (A00701-6) and anti-Beta Tubulin antibody (M01857-3).
GLI2 was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of U2OS cells using anti-GLI2 antibody (A00701-6).
Overlay histogram showing U2OS cells stained with A00701-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (A00701-6, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of anti-GLI2 antibody (A00701-6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (A00701-6) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 168 kDa.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GLI2 using anti-GLI2 antibody (A00701-6).
GLI2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GLI2 using anti-GLI2 antibody (A00701-6) and anti-Beta Tubulin antibody (M01857-3).
GLI2 was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of U2OS cells using anti-GLI2 antibody (A00701-6).
Overlay histogram showing U2OS cells stained with A00701-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (A00701-6, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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