Anti-GLI2 Antibody

Rabbit polyclonal antibody

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BA3326-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GLI2 using anti-GLI2 antibody (BA3326-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (BA3326-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 168 kDa.
all(2)
Western blot analysis of GLI2 using anti-GLI2 antibody (BA3326-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: SKOV whole cell lysates,
Lane 4: HT1080 whole cell lysates,
Lane 5: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (BA3326-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 168 kDa.
all(2)

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产品简介>

产品名称

Anti-GLI2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GLI family zinc finger 2; CJS; HPE9; PHS2; THP1; THP2; GLI2

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

GLI2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2.

计算分子量

168 kDa

实际分子量

180-200 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

GLI2(Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel(Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Uniprot ID

P10070

文献引用格式

GLI2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3326-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

GLI family zinc finger 2

蛋白名全称

Zinc finger protein GLI2

实验方案>

实验步骤

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