Anti-CD163 Antibody

筛选器： All WB FCM

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  Western blot analysis of CD163 using anti-CD163 antibody (A00812-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HCCT tissue lysates,
  Lane 2: HCCP tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (A00812-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD163 at approximately 125-150 kDa. The expected band size for CD163 is at 125 kDa.

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  Flow Cytometry analysis of Jurkat cells using anti-CD163 antibody (A00812-3).
  Overlay histogram showing Jurkat cells stained with A00812-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD163 Antibody (A00812-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of CD163 using anti-CD163 antibody (A00812-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HCCT tissue lysates,
  Lane 2: HCCP tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (A00812-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD163 at approximately 125-150 kDa. The expected band size for CD163 is at 125 kDa.

• 

  Flow Cytometry analysis of Jurkat cells using anti-CD163 antibody (A00812-3).
  Overlay histogram showing Jurkat cells stained with A00812-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD163 Antibody (A00812-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.