Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Western blot analysis of Angiopoietin-1/ANGPT1 using anti-Angiopoietin-1/ANGPT1 antibody (A00853).
Lane 1: recombinant human ANGPT1 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Angiopoietin-1/ANGPT1 antigen affinity purified polyclonal antibody (A00853) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Angiopoietin-1/ANGPT1 at approximately 70 kDa.
Western blot analysis of Angiopoietin-1/ANGPT1 using anti-Angiopoietin-1/ANGPT1 antibody (A00853).
Lane 1: recombinant human ANGPT1 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Angiopoietin-1/ANGPT1 antigen affinity purified polyclonal antibody (A00853) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Angiopoietin-1/ANGPT1 at approximately 70 kDa.