Anti-WNT4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00879

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of WNT4 using anti-WNT4 antibody (A00879). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-WNT4 antigen affinity purified polyclonal antibody (A00879) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for WNT4 at approximately 39 kDa. The expected band size for WNT4 is at 39 kDa.
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IHC analysis of WNT4 using anti-WNT4 antibody (A00879).
WNT4 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-WNT4 Antibody (A00879) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of WNT4 using anti-WNT4 antibody (A00879).
WNT4 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-WNT4 Antibody (A00879) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
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产品简介>

产品名称

Anti-WNT4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Protein Wnt 4; SERKAL; WNT 4; WNT4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

WNT4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human WNT4 recombinant protein (Position: K100-E274).

计算分子量

39 kDa

实际分子量

39 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Wingless-type MMIV integration site family, member4, is a secreted protein that in humans is encoded by the Wnt4 gene. By fluorescence in situ hybridization, WNT4 gene was mapped to chromosome 1p35, a location consistent with the results of radiation hybrid mapping. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis.

Uniprot ID

P56705

文献引用格式

WNT4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00879)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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