Western blot (WB): | 1:500-2000 |
Western blot analysis of WNT4 using anti-WNT4 antibody (BA3160-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-WNT4 antigen affinity purified polyclonal antibody (BA3160-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for WNT4 at approximately 39 kDa. The expected band size for WNT4 is at 39 kDa.
Western blot analysis of WNT4 using anti-WNT4 antibody (BA3160-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-WNT4 antigen affinity purified polyclonal antibody (BA3160-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for WNT4 at approximately 39 kDa. The expected band size for WNT4 is at 39 kDa.