Anti-p75NTR/NGFR Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of p75NTR/NGFR using anti-p75NTR/NGFR antibody (A01187). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human CACO-2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p75NTR/NGFR antigen affinity purified polyclonal antibody (A01187) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p75NTR/NGFR at approximately 45 kDa,75 kDa. The expected band size for p75NTR/NGFR is at 45 kDa.

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  IF analysis of p75NTR/NGFR using anti-p75NTR/NGFR antibody (A01187).
  p75NTR/NGFR was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-p75NTR/NGFR Antibody (A01187) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of A431 cells using anti-p75NTR/NGFR antibody (A01187).
  Overlay histogram showing A431 cells stained with A01187 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p75NTR/NGFR Antibody (A01187) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of p75NTR/NGFR using anti-p75NTR/NGFR antibody (A01187). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: human SW620 whole cell lysates,
  Lane 3: human CACO-2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p75NTR/NGFR antigen affinity purified polyclonal antibody (A01187) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p75NTR/NGFR at approximately 45 kDa,75 kDa. The expected band size for p75NTR/NGFR is at 45 kDa.

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  IF analysis of p75NTR/NGFR using anti-p75NTR/NGFR antibody (A01187).
  p75NTR/NGFR was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-p75NTR/NGFR Antibody (A01187) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of A431 cells using anti-p75NTR/NGFR antibody (A01187).
  Overlay histogram showing A431 cells stained with A01187 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p75NTR/NGFR Antibody (A01187) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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