Anti-HLA-DRA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A01195

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Ramos whole cell lysates,
Lane 4: human HMy2,CIR whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified polyclonal antibody (A01195) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLA-DRA at approximately 36 kDa. The expected band size for HLA-DRA is at 29 kDa.
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IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
HLA-DRA was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
HLA-DRA was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
HLA-DRA was detected in a paraffin-embedded section of human duodenal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
HLA-DRA was detected in a paraffin-embedded section of human seminoma testis tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Flow cytometry analysis of h_PBMC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介>

产品名称

Anti-HLA-DRA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HLA DRA; HLA DRA1; HLA-DR; HLA-DRA; MHC class II antigen DRA

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, FCM, ELISA

基因名称

HLA-DRA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human HLA-DR/HLA-DRA recombinant protein (Position: I26-L254).

计算分子量

29 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

HLA class II histocompatibility antigen, DR alpha chain is a protein that in humans is encoded by the HLA-DRA gene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Uniprot ID

P01903

文献引用格式

HLA-DRA Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01195)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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