Western blot analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HT1080 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human SK-OV-3 whole cell lysates,
Lane 6: Monkey COS-7 whole cell lysates,
Lane 7: human K562 whole cell lysates,
Lane 8: human SGC-7901 whole cell lysates,
Lane 9: Rat heart tissue lysates,
Lane 10: Mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vinculin/VCL antigen affinity purified polyclonal antibody (A01207-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vinculin/VCL at approximately 124 kDa. The expected band size for Vinculin/VCL is at 124 kDa.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti-VCL antibody (A01207-1). detected in paraffin-embedded section of human breastcancer tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(6) 
Flow Cytometry analysis of Hela cells using anti-Vinculin/VCL antibody (A01207-1).
Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HT1080 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human SK-OV-3 whole cell lysates,
Lane 6: Monkey COS-7 whole cell lysates,
Lane 7: human K562 whole cell lysates,
Lane 8: human SGC-7901 whole cell lysates,
Lane 9: Rat heart tissue lysates,
Lane 10: Mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vinculin/VCL antigen affinity purified polyclonal antibody (A01207-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vinculin/VCL at approximately 124 kDa. The expected band size for Vinculin/VCL is at 124 kDa.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti-VCL antibody (A01207-1). detected in paraffin-embedded section of human breastcancer tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of Hela cells using anti-Vinculin/VCL antibody (A01207-1).
Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HT1080 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human SK-OV-3 whole cell lysates,
Lane 6: Monkey COS-7 whole cell lysates,
Lane 7: human K562 whole cell lysates,
Lane 8: human SGC-7901 whole cell lysates,
Lane 9: Rat heart tissue lysates,
Lane 10: Mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vinculin/VCL antigen affinity purified polyclonal antibody (A01207-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vinculin/VCL at approximately 124 kDa. The expected band size for Vinculin/VCL is at 124 kDa.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vinculin/VCL using anti-Vinculin/VCL antibody (A01207-1).
Vinculin/VCL was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti-VCL antibody (A01207-1). detected in paraffin-embedded section of human breastcancer tissue. The tissue section were stained using the Dylight488 conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of Hela cells using anti-Vinculin/VCL antibody (A01207-1).
Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Vinculin/VCL Antibody (A01207-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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