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Anti-EPAS1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB FCM

A01248-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of EPAS1 using anti-EPAS1 antibody (A01248-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Rat lung tissue lysates,
    Lane 2: Rat PC-12 whole cell lysates,
    Lane 3: Mouse lung tissue lysates,
    Lane 4: Mouse SP2/0 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPAS1 antigen affinity purified polyclonal antibody (A01248-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPAS1 at approximately 96 kDa. The expected band size for EPAS1 is at 96 kDa.

    all(2)
  • Flow Cytometry analysis of SiHa cells using anti-EPAS1 antibody (A01248-1).
    Overlay histogram showing SiHa cells stained with A01248-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPAS1 Antibody (A01248-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    all(2)

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产品名称
Anti-EPAS1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
bHLHe73; ECYT4; EPAS 1; EPAS1; HIF 1 alpha like factor; HIF 2 alpha; HIF2 alpha; HIF2A; HIF-2A; HIF2α; HIF-2α; HLF; Member of PAS protein 2; MOP2; PASD2
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, FCM
基因名称
EPAS1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human HIF-2-alpha/EPAS1, which shares 94.4% amino acid (aa) sequence identity with both mouse and rat HIF-2-alpha/EPAS1.
计算分子量
96 kDa
实际分子量
96 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.
Uniprot ID
Q99814  
RRID
文献引用格式
EPAS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01248-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
基因名全称
endothelial PAS domain protein 1
蛋白名全称
Endothelial PAS domain-containing protein 1
推荐稀释比
Western blot (WB):1:500-2000
Flow Cytometry (Fixed):1:50-200

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    Anti-EPAS1 Antibody

    筛选器: All WB FCM

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