Western blot analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR3/FLT4 antigen affinity purified polyclonal antibody (A01276-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFR3/FLT4 at approximately 153 kDa. The expected band size for VEGFR3/FLT4 is at 153 kDa.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of VEGF Receptor 3 using anti- VEGF Receptor 3 antibody (A01276-2).
VEGF Receptor 3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of U2OS cells using anti- VEGF Receptor 3 antibody (A01276-2). Overlay histogram showing U2OS cells stained with A01276-2 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(10) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR3/FLT4 antigen affinity purified polyclonal antibody (A01276-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFR3/FLT4 at approximately 153 kDa. The expected band size for VEGFR3/FLT4 is at 153 kDa.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of VEGF Receptor 3 using anti- VEGF Receptor 3 antibody (A01276-2).
VEGF Receptor 3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of U2OS cells using anti- VEGF Receptor 3 antibody (A01276-2). Overlay histogram showing U2OS cells stained with A01276-2 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VEGFR3/FLT4 antigen affinity purified polyclonal antibody (A01276-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VEGFR3/FLT4 at approximately 153 kDa. The expected band size for VEGFR3/FLT4 is at 153 kDa.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of VEGFR3/FLT4 using anti-VEGFR3/FLT4 antibody (A01276-2).
VEGFR3/FLT4 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VEGFR3/FLT4 Antibody (A01276-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of VEGF Receptor 3 using anti- VEGF Receptor 3 antibody (A01276-2).
VEGF Receptor 3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Flow Cytometry analysis of U2OS cells using anti- VEGF Receptor 3 antibody (A01276-2). Overlay histogram showing U2OS cells stained with A01276-2 (Blue line). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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