Anti-Cannabinoid receptor 1/CNR1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A01291-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cannabinoid receptor 1/CNR1 using anti-Cannabinoid receptor 1/CNR1 antibody (A01291-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cannabinoid receptor 1/CNR1 antigen affinity purified polyclonal antibody (A01291-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cannabinoid receptor 1/CNR1 at approximately 53-60 kDa. The expected band size for Cannabinoid receptor 1/CNR1 is at 53 kDa.
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IHC analysis of Cannabinoid receptor 1/CNR1 using anti-Cannabinoid receptor 1/CNR1 antibody (A01291-1).
Cannabinoid receptor 1/CNR1 was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cannabinoid receptor 1/CNR1 Antibody (A01291-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Cannabinoid receptor 1/CNR1 using anti-Cannabinoid receptor 1/CNR1 antibody (A01291-1).
Cannabinoid receptor 1/CNR1 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cannabinoid receptor 1/CNR1 Antibody (A01291-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of Hela cells using anti-Cannabinoid receptor 1/CNR1 antibody (A01291-1).
Overlay histogram showing Hela cells stained with A01291-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cannabinoid receptor 1/CNR1 Antibody (A01291-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-Cannabinoid receptor 1/CNR1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CANN6; Cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB R; CB1; CB1A; CB1K5; CB1R; CNR; CNR1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

CNR1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Cannabinoid Receptor I recombinant protein (Position: M1-Q75). Human Cannabinoid Receptor I shares 97.3% amino acid (aa) sequence identity with both mouse and rat Cannabinoid Receptor I.

计算分子量

53 kDa

实际分子量

53-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The cannabinoid receptor type 1, often abbreviated as CB1, is a G protein-coupled cannabinoid receptor located primarily in the central and peripheral nervous system. This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Uniprot ID

P21554

文献引用格式

Cannabinoid receptor 1/CNR1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01291-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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