Anti-ABCG8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF

A01482-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEPG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: PC-12 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (A01482-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCG8 at approximately 76 kDa. The expected band size for ABCG8 is at 76 kDa.
all(2)
ICC/IF analysis of ABCG8 using anti-ABCG8 antibody (A01482-1).
ABCG8 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-ABCG8 Antibody (A01482-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(2)

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产品简介>

产品名称

Anti-ABCG8 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Sterolin-2; ABCG8; GBD4; STSL; STSL1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

ABCG8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human ABCG8, different from the related mouse and rat sequences by twelve amino acids.

计算分子量

76 kDa

实际分子量

76 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ATP-binding cassette sub-family G member 8 is a protein that in humans is encoded by the ABCG8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.

Uniprot ID

Q9H221

文献引用格式

ABCG8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01482-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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