Anti-N-Cadherin/CDH2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF FCM ELISA

A01577-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Mouse liver tissue lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified polyclonal antibody (A01577-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for N-Cadherin/CDH2 at approximately 140 kDa. The expected band size for N-Cadherin/CDH2 is at 100 kDa.
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IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3).
N-Cadherin/CDH2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis using anti- CDH2 antibody (A01577-3). detected in paraffin-embedded section of human liver cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
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IF analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (A01577-3) and anti-Beta Tubulin antibody (M01857-3).
N-Cadherin/CDH2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of HepG2 cells using anti-N-Cadherin/CDH2 antibody (A01577-3).
Overlay histogram showing HepG2 cells stained with A01577-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N-Cadherin/CDH2 Antibody (A01577-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-N-Cadherin/CDH2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cadherin 2; CD325; CDH2; CDHN; CDw325; N cadherin; NCAD; N-cadherin; Neural cadherin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, FCM, ELISA

基因名称

CDH2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human N Cadherin/CDH2 recombinant protein (Position: E170-E266). Human CDH2 shares 99% amino acid (aa) sequence identity with both mouse and rat CDH2.

计算分子量

100 kDa

实际分子量

140 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Uniprot ID

P19022

RRID

AB_3081461

文献引用格式

N-Cadherin/CDH2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01577-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cadherin 2

蛋白名全称

Cadherin-2

实验方案>

实验步骤

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