Anti-14-3-3 Epsilon/YWHAE Antibody

筛选器： All WB IHC FCM ICC/IF ELISA

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  Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human hepg2 whole cell lysates,
  Lane 4: human SH-SY5Y whole cell lysates,
  Lane 5: human HEK293 whole cell lysates,
  Lane 6: human SW620 whole cell lysates,
  Lane 7: human A549 whole cell lysates,
  Lane 8: human Raji whole cell lysates,
  Lane 9: Rat brain tissue lysates,
  Lane 10: Mouse brain tissue lysates,
  Lane 11: Mouse NIH/3T3 whole cell lysates,
  Lane 12: Mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody (A01687-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of A549 cells using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  Overlay histogram showing A549 cells stained with A01687-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human hepg2 whole cell lysates,
  Lane 4: human SH-SY5Y whole cell lysates,
  Lane 5: human HEK293 whole cell lysates,
  Lane 6: human SW620 whole cell lysates,
  Lane 7: human A549 whole cell lysates,
  Lane 8: human Raji whole cell lysates,
  Lane 9: Rat brain tissue lysates,
  Lane 10: Mouse brain tissue lysates,
  Lane 11: Mouse NIH/3T3 whole cell lysates,
  Lane 12: Mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody (A01687-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

• 

  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of A549 cells using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  Overlay histogram showing A549 cells stained with A01687-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (A01687-4).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody (A01687-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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