Anti-Cardiac Troponin I/TNNI3 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB

A01720-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (A01720-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 28 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa.
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产品简介>

产品名称

Anti-Cardiac Troponin I/TNNI3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Cardiac troponin I; CMD2A; CMH7; cTnI; RCM1; TNNC1; TNNI3; troponin I type 3 (cardiac); Troponin I; cardiac muscle

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

基因名称

TNNI3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Cardiac Troponin I/TNNI3, which shares 95.5% amino acid (aa) sequence identity with mouse and rat TNNI3.

计算分子量

24 kDa

实际分子量

28 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Troponin I, cardiac muscle is a protein that in humans is encoded by the TNNI3 gene. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.

Uniprot ID

P19429

文献引用格式

Cardiac Troponin I/TNNI3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01720-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

troponin I3, cardiac type

蛋白名全称

Troponin I, cardiac muscle

实验方案>

实验步骤

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