| Western blot (WB): | 1:500-2000 |
| ELISA: | 1:100-1000 |

Western blot analysis of PER3 using anti-PER3 antibody (A01835-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human COLO-320 whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PER3 antigen affinity purified polyclonal antibody (A01835-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PER3 at approximately 132 kDa. The expected band size for PER3 is at 132 kDa
.

Western blot analysis of PER3 using anti-PER3 antibody (A01835-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human COLO-320 whole cell lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PER3 antigen affinity purified polyclonal antibody (A01835-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PER3 at approximately 132 kDa. The expected band size for PER3 is at 132 kDa
.


