Anti-C5AR1 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of C5AR1 using anti-C5AR1 antibody (A01898-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Mouse spleen tissue lysates,
  Lane 2: Mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C5AR1 antigen affinity purified polyclonal antibody (A01898-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C5AR1 at approximately 43 kDa. The expected band size for C5AR1 is at 43 kDa.

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  IF analysis of C5AR1 using anti-C5AR1 antibody (A01898-2).
  C5AR1 was detected in an immunocytochemical section of Hepa1-6 cells. The section was incubated with rabbit anti-C5AR1 Antibody (A01898-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Raw264.7 cells using anti-C5AR1 antibody (A01898-2).
  Overlay histogram showing Raw264.7 cells stained with A01898-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C5AR1 Antibody (A01898-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of C5AR1 using anti-C5AR1 antibody (A01898-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Mouse spleen tissue lysates,
  Lane 2: Mouse thymus tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C5AR1 antigen affinity purified polyclonal antibody (A01898-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C5AR1 at approximately 43 kDa. The expected band size for C5AR1 is at 43 kDa.

• 

  IF analysis of C5AR1 using anti-C5AR1 antibody (A01898-2).
  C5AR1 was detected in an immunocytochemical section of Hepa1-6 cells. The section was incubated with rabbit anti-C5AR1 Antibody (A01898-2) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Raw264.7 cells using anti-C5AR1 antibody (A01898-2).
  Overlay histogram showing Raw264.7 cells stained with A01898-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C5AR1 Antibody (A01898-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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