Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of anti- TWEAK/Tnfsf12 antibody (A02009-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
Use rabbit anti- TWEAK/Tnfsf12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TWEAK/Tnfsf12 at approximately 27KD. The expected band size for TWEAK/Tnfsf12 is at 22KD.
Western blot analysis of anti- TWEAK/Tnfsf12 antibody (A02009-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
Use rabbit anti- TWEAK/Tnfsf12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TWEAK/Tnfsf12 at approximately 27KD. The expected band size for TWEAK/Tnfsf12 is at 22KD.