Anti-HCN4 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of HCN4 using anti-HCN4 antibody (A02235-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HCN4 antigen affinity purified polyclonal antibody (A02235-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HCN4 at approximately 140 kDa. The expected band size for HCN4 is at 129 kDa.

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  Flow Cytometry analysis of MCF-7 cells using anti-HCN4 antibody (A02235-1).
  Overlay histogram showing MCF-7 cells stained with A02235-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCN4 Antibody (A02235-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of HCN4 using anti-HCN4 antibody (A02235-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HCN4 antigen affinity purified polyclonal antibody (A02235-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HCN4 at approximately 140 kDa. The expected band size for HCN4 is at 129 kDa.

• 

  Flow Cytometry analysis of MCF-7 cells using anti-HCN4 antibody (A02235-1).
  Overlay histogram showing MCF-7 cells stained with A02235-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCN4 Antibody (A02235-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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