欢迎访问博士德生物官网! 服务热线:027-67845390 |
您当前的位置: 首页 > 产品中心 > 一抗 > 多克隆抗体

Anti-Cytokeratin 17/KRT17-Specific Antibody

Rabbit polyclonal antibody

说明书 一键复制产品信息

筛选器: All WB IHC ICC/IF IP FCM ELISA

A02289-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Cytokeratin 17/KRT17 using anti-Cytokeratin 17/KRT17 antibody (A02289-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human A431 whole cell lysates,
    Lane 3: human HepG2 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: rat PC-12 whole cell lysates,
    Lane 6: rat NRK whole cell lysates,
    Lane 7: mouse NIH/3T3 whole cell lysates,
    Lane 8: mouse SP2/0 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 17/KRT17 antigen affinity purified polyclonal antibody (A02289-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 17/KRT17 at approximately 48 kDa. The expected band size for Cytokeratin 17/KRT17 is at 48 kDa.

    all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-Cytokeratin 17/KRT17-Specific Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
39.1; Cell and organelle markers; CK 17; Cytokeratin 17; Cytokeratin 17-Specific; Cytoskeleton Marker; K17; keratin 17; KRT17; PCHC1
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, IP, FCM, ELISA
基因名称
KRT17
宿主
Rabbit
抗体亚型
IgG
计算分子量
48 kDa
实际分子量
48 kDa
成分
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.
Uniprot ID
Q04695  
文献引用格式
Cytokeratin 17/KRT17-Specific Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02289-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
Keratin, type I cytoskeletal 17
蛋白名全称
Keratin, type I cytoskeletal 17
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
ImmunoPrecipitation (IP):1:50
Flow Cytometry (FCM):1-3 μg/1x106 cells
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

实验步骤

引用文献(0)>

    相关产品>

    成功添加到购物车

    查看购物车 继续购物

    Anti-Cytokeratin 17/KRT17-Specific Antibody

    筛选器: All WB IHC ICC/IF IP FCM ELISA

    亮暗模式切换
    X
    亮暗模式切换
    X

    在线
    客服

    在线客服
    服务时间:08:30 - 17:30

    点击下列客服在线咨询:

    服务热线

    服务
    热线

    客服服务热线

    027-67845390
    客服服务热线

    扫码

    扫码
    咨询

    微信二维码 微信扫一扫立即咨询
    返回顶部 顶部