Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (A02429-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human stomach cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRP3/ABCC3 antigen affinity purified polyclonal antibody (A02429-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MRP3/ABCC3 at approximately 200 kDa. The expected band size for MRP3/ABCC3 is at 169 kDa.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MRP3/ABCC3 using anti-MRP3/ABCC3 antibody (A02429-1).
MRP3/ABCC3 was detected in a paraffin-embedded section of human stomach cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MRP3/ABCC3 Antibody (A02429-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.