Anti-XRN1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A02855-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of XRN1 using anti-XRN1 antibody (A02855-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XRN1 antigen affinity purified polyclonal antibody (A02855-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for XRN1 at approximately 194 kDa. The expected band size for XRN1 is at 194 kDa.
all(3)
ICC/IF analysis of XRN1 using anti-XRN1 antibody (A02855-2).
XRN1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-XRN1 Antibody (A02855-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品简介>

产品名称

Anti-XRN1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

XRN1; SEP1; 5'-3' exoribonuclease 1; Strand-exchange protein 1 homolog

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, ICC/IF, ELISA

基因名称

XRN1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human XRN1 recombinant protein (Position: K523-H1198). Human XRN1 shares 93.2% amino acid (aa) sequence identity with mouse XRN1.

计算分子量

194 kDa

实际分子量

194 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

5′-3′ exoribonuclease 1 (Xrn1) is a protein that in humans is encoded by the XRN1 gene. This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants.

Uniprot ID

Q8IZH2

Gene ID

54464

文献引用格式

XRN1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02855-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

5'-3' exoribonuclease 1

蛋白名全称

5'-3' exoribonuclease 1

实验方案>

实验步骤

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