Anti-CAMKK2 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ICC/IF FCM ELISA

A03048

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CAMKK2 using anti-CAMKK2 antibody (A03048). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CAMKK2 antigen affinity purified polyclonal antibody (A03048) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CAMKK2 at approximately 65 kDa. The expected band size for CAMKK2 is at 65 kDa.
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IHC analysis of CAMKK2 using anti-CAMKK2 antibody (A03048) .
CAMKK2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CAMKK2 using anti-CAMKK2 antibody (A03048) .
CAMKK2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CAMKK2 using anti-CAMKK2 antibody (A03048) .
CAMKK2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CAMKK2 using anti-CAMKK2 antibody (A03048) .
CAMKK2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CAMKK2 using anti-CAMKK2 antibody (A03048) .
CAMKK2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of CAMKK2 using anti-CAMKK2 antibody (A03048).
CAMKK2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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ICC/IF analysis of CAMKK2 using anti-CAMKK2 antibody (A03048).
CAMKK2 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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ICC/IF analysis of CAMKK2 using anti-CAMKK2 antibody (A03048).
CAMKK2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CAMKK2 Antibody (A03048) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CAMKK2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CaM kinase kinase 2; CaM kinase kinase beta; CaM KK 2; CaM KK beta; CAMKK; CaMKK 2; CaMKK beta; CAMKK2; CAMKKB; KIAA0787

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, FCM, ELISA

基因名称

CAMKK2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CAMKK2 recombinant protein (Position: M1-G349).

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.

Uniprot ID

Q96RR4

文献引用格式

CAMKK2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03048)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

calcium/calmodulin-dependent protein kinase kinase 2, beta

蛋白名全称

Calcium/calmodulin-dependent protein kinase kinase 2(CaM-KK 2/CaM-kinase kinase 2/CaMKK 2)

实验方案>

实验步骤

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