Anti-ANLN Antibody

筛选器： All WB ICC/IF IP FCM

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  Western blot analysis of ANLN using anti-ANLN antibody (A03997-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: rat PC-12 whole cell lysates,
  Lane 4: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANLN antigen affinity purified polyclonal antibody (A03997-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANLN at approximately 150 kDa. The expected band size for ANLN is at 124 kDa.

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  IF analysis of ANLN using anti-ANLN antibody (A03997-1) and anti-Beta Tubulin antibody (M01857-3).
  ANLN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ANLN Antibody (A03997-1) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

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  IP analysis of ANLN using anti-ANLN antibody (A03997-1) in U251 whole cell lysate.
  Western blot analysis of ANLN using anti- ANLN antibody (A03997-1).
  Lane 1: U251 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- ANLN antibody in U251 whole cell lysate,
  Lane 3: anti- ANLN antibody (2μg) + U251 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ANLN antigen affinity purified polyclonal antibody (A03997-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANLN at approximately 150 kDa. The expected band size for ANLN is at 124 kDa.

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  Flow Cytometry analysis of U251 cells using anti-ANLN antibody (A03997-1).
  Overlay histogram showing U251 cells stained with A03997-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANLN Antibody (A03997-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ANLN using anti-ANLN antibody (A03997-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: rat PC-12 whole cell lysates,
  Lane 4: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANLN antigen affinity purified polyclonal antibody (A03997-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANLN at approximately 150 kDa. The expected band size for ANLN is at 124 kDa.

• 

  IF analysis of ANLN using anti-ANLN antibody (A03997-1) and anti-Beta Tubulin antibody (M01857-3).
  ANLN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ANLN Antibody (A03997-1) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

• 

  IP analysis of ANLN using anti-ANLN antibody (A03997-1) in U251 whole cell lysate.
  Western blot analysis of ANLN using anti- ANLN antibody (A03997-1).
  Lane 1: U251 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- ANLN antibody in U251 whole cell lysate,
  Lane 3: anti- ANLN antibody (2μg) + U251 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ANLN antigen affinity purified polyclonal antibody (A03997-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ANLN at approximately 150 kDa. The expected band size for ANLN is at 124 kDa.

• 

  Flow Cytometry analysis of U251 cells using anti-ANLN antibody (A03997-1).
  Overlay histogram showing U251 cells stained with A03997-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANLN Antibody (A03997-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.