Anti-15 Lipoxygenase 2/ALOX15B Antibody
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Rabbit polyclonal antibody

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筛选器： All WB ELISA

A04331-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of 15 Lipoxygenase 2/ALOX15B using anti-15 Lipoxygenase 2/ALOX15B antibody (A04331-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-15 Lipoxygenase 2/ALOX15B antigen affinity purified polyclonal antibody (A04331-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 15 Lipoxygenase 2/ALOX15B at approximately 70 kDa. The expected band size for 15 Lipoxygenase 2/ALOX15B is at 76 kDa.
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产品简介>

产品名称

Anti-15 Lipoxygenase 2/ALOX15B Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

15 Lipoxygenase 2; 15 LOX 2; 15 LOX B; ALOX15B; ALOX8; Arachidonate 15 lipoxygenase B

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

ALOX15B

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human 15 Lipoxygenase 2/ALOX15B recombinant protein (Position: L38-K582).

计算分子量

76 kDa

实际分子量

70 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Uniprot ID

O15296

Gene ID

247

文献引用格式

15 Lipoxygenase 2/ALOX15B Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04331-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

arachidonate 15-lipoxygenase type B

蛋白名全称

Polyunsaturated fatty acid lipoxygenase ALOX15B

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实验步骤

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